rsbU [2019-09-03 12:04:37]

protein serine phosphatase, environmental PP2C, dephosphorylates RsbV

Locus: BSU_04700

Isoelectric point: 4.91

Molecular weight: 38.48 kDa

Protein length: 335 aa

Gene length: 1008 bp

Function: control of SigB activity

Product: protein serine phosphatase, environmental PP2C

Essential: no

E.C.: 3.1.3.3

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein phosphatases
• Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors

Gene

Coordinates: 521,019 522,026

The protein

Catalyzed reaction/ biological activity

• O-phospho-L(or D)-serine + H₂O --> L(or D)-serine + phosphate (according to UniProt)

Structure

• 2J6Y

Expression and Regulation

Operons

• Genes: rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • rsbV: induced by stress (SigB) PubMed

Biological materials

Mutant

• BKE04700 (rsbU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCTAAAATCCACGTTCTTAC, downstream forward: _UP4_TAACGTCTGTCAGACGAGGG
• BKK04700 (rsbU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCTAAAATCCACGTTCTTAC, downstream forward: _UP4_TAACGTCTGTCAGACGAGGG

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage
• Rick Lewis, Newcastle, UK homepage

References

Reviews

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Original publications

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